abstract：:The behavior of 125I-labeled recombinant hirudin towards the uninjured and de-endothelialized rabbit aorta wall has been studied in vitro and in vivo to determine its usefulness as an indicator of thrombin activity associated with the aorta wall. Thrombin adsorbed to either sulfopropyl-Sephadex or heparin-Sepharose bo...

journal_title：Thrombosis and haemostasis

authors： Hatton MW,Ross-Ouellet B

更新日期：1994-04-01 00:00:00

abstract：:The effects on the haemoglobin level of low dose aspirin and of low intensity oral anticoagulation with warfarin separately and in combination have been established in men aged between 45 and 69 at high risk of ischaemic heart disease. The findings confirm that combined treatment with warfarin and aspirin (WA) leads t...

journal_title：Thrombosis and haemostasis

authors： Meade TW,Howarth DJ,Brennan PJ

更新日期：1994-03-01 00:00:00

abstract：:Clots formed from platelet rich plasma were found to be lysed more readily by low concentrations of pro-urokinase (pro-UK) than clots formed from platelet poor plasma. This was not a non-specific effect since the reverse occurred with tissue plasminogen activator. A mechanical explanation due to platelet-mediated clot...

journal_title：Thrombosis and haemostasis

authors： Loza JP,Gurewich V,Johnstone M,Pannell R

更新日期：1994-03-01 00:00:00

abstract：:We have developed a sensitive and quantitative RT-PCR assay for the determination of tissue factor (TF) mRNA levels in human cells. An in vitro synthesized internal standard RNA was used to correct for differences in reverse transcription or amplification of various RNA samples. The PCR products were quantitated by hy...

journal_title：Thrombosis and haemostasis

authors： Pötgens AJ,Lubsen NH,van Altena G,Schoenmakers JG,Ruiter DJ,de Waal RM

更新日期：1994-02-01 00:00:00

abstract：:Platelet-rich clots are inefficiently lysed by current fibrinolytic agents. Platelets contain a great deal of plasminogen activator inhibitor 1 (PAI-1), the principal endogenous inhibitor of tissue-type plasminogen activator (t-PA). We have tested whether PAI-1 resistant t-PAs would be more effective thrombolytic agen...

journal_title：Thrombosis and haemostasis

authors： Shohet RV,Spitzer S,Madison EL,Bassel-Duby R,Gething MJ,Sambrook JF

更新日期：1994-01-01 00:00:00

abstract：:The effect of shear rate and fibrinogen concentration on adenosine diphosphate-induced aggregation of suspensions of washed human platelets in Poiseuille flow at 23 degrees C was studied using a previously described double infusion technique and resistive particle counter size analysis. Using suspensions of multiple-c...

journal_title：Thrombosis and haemostasis

authors： Goldsmith HL,Frojmovic MM,Braovac S,McIntosh F,Wong T

更新日期：1994-01-01 00:00:00

abstract：:Biosynthesis of the adhesive glycoprotein von Willebrand factor (vWf) by endothelial cells results in constitutive secretion of small multimers and storage of the largest multimers in rod-shaped granules called Weibel-Palade bodies. This pattern is reproduced by expression of pro-vWf in heterologous cells with a regul...

journal_title：Thrombosis and haemostasis

authors： Journet AM,Saffaripour S,Wagner DD

更新日期：1993-12-20 00:00:00

abstract：:Fibrin D-Dimer (D-Di), prothrombin activation fragment (F 1+2) and thrombin-antithrombin III complexes (TAT) were measured using ELISA procedures in the plasma of patients with an acute deep venous thrombosis (DVT), at presentation and on days 2, 6 and 10 after initiation of heparin treatment. Patients were randomly a...

journal_title：Thrombosis and haemostasis

authors：

更新日期：1993-12-20 00:00:00

abstract：:Tissue factor (TF) markedly enhances the ability of factor VIIa (FVIIa) to cleave both macromolecular and small peptidyl substrates. Using soluble mutant TF (sTF) to investigate TF-enhanced FVIIa amidolytic activity in solution, we screened thirty-four commercially available peptidyl-p-nitroanilide substrates and foun...

journal_title：Thrombosis and haemostasis

authors： Neuenschwander PF,Branam DE,Morrissey JH

更新日期：1993-12-20 00:00:00

abstract：:Thirty-one non-hemophilic patients with acquired antibodies to factor VIII were entered into a prospective, randomized, multi-institutional trial to determine the safety and efficacy of prednisone (P), cyclophosphamide (C), or the combination in the treatment of this disorder. Patients were eligible if they had antibo...

journal_title：Thrombosis and haemostasis

authors： Green D,Rademaker AW,Briët E

更新日期：1993-11-15 00:00:00

abstract：:Extensive contact between blood and artificial surfaces causes platelet activation and depletion. The aim of the present study was to test the efficacy of Ro 44-9883, a potent and selective peptidomimetic GPIIb-IIIa antagonist, in preventing platelet loss in guinea pigs undergoing extracorporeal circulation (ECC) with...

journal_title：Thrombosis and haemostasis

authors： Carteaux JP,Steiner B,Roux S

更新日期：1993-11-15 00:00:00

abstract：:Laboratory diagnosis of antiphospholipid antibodies is important in patients with clinical features of the antiphospholipid syndrome, such as thrombosis and fetal loss. We have developed a novel method for the detection of antiphospholipid antibodies using flow cytometry. Anionic phospholipids cardiolipin, phosphatidy...

journal_title：Thrombosis and haemostasis

authors： Stewart MW,Etches WS,Russell AS,Percy JS,Johnston CA,Chew CK,Gordon PA

更新日期：1993-10-18 00:00:00

abstract：:To assess interobserver variability of venography for screening for postoperative deep venous thrombosis (DVT), we used 185 bilateral ascending contrast venograms (366 lower limbs) which were performed using the long-leg film technique, in the frame of a trial of the efficacy of two low-molecular-weight-heparin fracti...

journal_title：Thrombosis and haemostasis

authors： Couson F,Bounameaux C,Didier D,Geiser D,Meyerovitz MF,Schmitt HE,Schneider PA,Bounameaux H

更新日期：1993-10-18 00:00:00

abstract：:We report genetic abnormalities of protein C gene in a male infant who developed neonatal purpura fulminans. DNA-sequence analysis of all exons in protein C gene in this family revealed two mutations. The first abnormality, derived from the mother, was a deletion of one of four consecutive G at nucleotide number 10758...

journal_title：Thrombosis and haemostasis

authors： Ido M,Ohiwa M,Hayashi T,Nishioka J,Hatada T,Watanabe Y,Wada H,Shirakawa S,Suzuki K

更新日期：1993-10-18 00:00:00

abstract：:The structural determinants of lipopolysaccharide required for the induction of tissue factor in human umbilical vein endothelial cells were studied. Intact lipid A was essential for the induction of tissue factor whereas the incomplete lipid A precursors lipid IVA and lipid X, as well as monophosphoryl lipid A and ac...

journal_title：Thrombosis and haemostasis

authors： Moldow CF,Bach RR,Staskus K,Rick PD

更新日期：1993-10-18 00:00:00

abstract：:The effect of supernatant from phorbol myristate acetate (PMA) stimulated human polymorphonuclear granulocytes (PMN) on human factor VII was studied in vitro. The supernatant caused a rapid loss in factor VII coagulant activity by the action of human leukocyte elastase (HLE) and cathepsin G in the supernatant, as demo...

journal_title：Thrombosis and haemostasis

authors： Anderssen T,Halvorsen H,Bajaj SP,Osterud B

更新日期：1993-09-01 00:00:00

abstract：:Recombinant human factor VIIa (rFVIIa; NovoSeven) is a two-chain activated clotting factor that is used in the treatment of haemophilia. The distribution of radioactivity in male and pregnant and non-pregnant female rats has been examined by whole-body autoradiography (WBA) after single intravenous doses of 125I-radio...

journal_title：Thrombosis and haemostasis

authors： Beeby TL,Chasseaud LF,Taylor T,Thomsen MK

更新日期：1993-09-01 00:00:00

abstract：:Platelet aggregation and fibrinogen binding in whole blood, induced either by ADP or by a 14 amino acid peptide mimicking an N-terminal region of the platelet thrombin receptor (TRP, thrombin receptor activating peptide), have been studied with blood from different species. Aggregation was assessed by counting the num...

journal_title：Thrombosis and haemostasis

authors： Cook NS,Zerwes HG,Tapparelli C,Powling M,Singh J,Metternich R,Hagenbach A

更新日期：1993-09-01 00:00:00

abstract：:Activation of coagulation was studied during the peri-operative period in patients undergoing cardiopulmonary bypass (CPB) surgery using activation markers which have recently become available: prothrombin fragment F1 + 2 (F1 + 2), which is a measure of total thrombin generation, and thrombin-antithrombin complex, whi...

journal_title：Thrombosis and haemostasis

authors： Boisclair MD,Lane DA,Philippou H,Sheikh S,Hunt B

更新日期：1993-08-02 00:00:00

abstract：:Site directed mutagenesis was used to construct a t-PA variant that contains an additional glycosylation site in the first kringle domain (T103N) combined with a tetra-alanine substitution in the protease domain (KHRR 296-299 AAAA). This combination variant has a plasma clearance rate that is 4.5-fold slower in rats a...

journal_title：Thrombosis and haemostasis

authors： Paoni NF,Keyt BA,Refino CJ,Chow AM,Nguyen HV,Berleau LT,Badillo J,Peña LC,Brady K,Wurm FM

更新日期：1993-08-02 00:00:00

abstract：:The stimulated release of von Willebrand factor (vWF) from endothelial cells by secretagogues such as thrombin is associated with the translocation of Weibel-Palade bodies to the cell membrane and the surface expression of P-selectin (also known as GMP 140, PADGEM and CD 62). P-selectin, which is stored in Weibel-Pala...

journal_title：Thrombosis and haemostasis

authors： Collins PW,Macey MG,Cahill MR,Newland AC

更新日期：1993-08-02 00:00:00

abstract：:The surface of the platelet expresses procoagulant phospholipids that bind coagulation factors and contribute to the procoagulant activity of the cell. The evidence, however, also suggests that platelets may possess specific receptors for coagulation Factors V(a) and VIII(a). Examination of a limited set of the availa...

journal_title：Thrombosis and haemostasis

authors： Nesheim ME,Furmaniak-Kazmierczak E,Henin C,Côté G

更新日期：1993-07-01 00:00:00

abstract：:Vascular endothelial cells perform many differentiated functions in processes such as angiogenesis, hemostasis, and inflammation. The number of recognized differentiated functions has increased rapidly in recent years, but there may be many more still unrecognized. The purpose of this study is to estimate the fraction...

journal_title：Thrombosis and haemostasis

authors： Rieber AJ,Marr HS,Comer MB,Edgell CJ

更新日期：1993-05-03 00:00:00

abstract：:We have investigated the influence of long term oral anticoagulants (OAC) upon the plasma levels of prothrombin fragment 1 + 2 (F1 + 2), of thrombin-antithrombin III complexes (TAT) and of D-Dimer in 20 patients affected by a proximal deep vein thrombosis (DVT) diagnosed by ultrasonic duplex scanning. Patients (63 +/-...

journal_title：Thrombosis and haemostasis

authors： Elias A,Bonfils S,Daoud-Elias M,Gauthier B,Sié P,Boccalon H,Boneu B

更新日期：1993-04-01 00:00:00

abstract：:Heparin is often used as an adjunct to thrombolytic therapy in order to prevent reocclusion of the patent vessels in patients with thrombotic disease. Controversy exists as to whether heparin is required for effective clot lysis with tissue-type plasminogen activator, while in vitro data and small scale clinical trial...

journal_title：Thrombosis and haemostasis

authors： Burke SE,Lubbers NL,Nelson RA,Henkin J

更新日期：1993-04-01 00:00:00

abstract：:Anisoylated Lys-plasminogen streptokinase activator complex (APSAC) was purified from Eminase by chromatography on Superose-12. Purified APSAC did not significantly deacylate within 4 h at 4 degrees C in solution as determined by hydrolysis of D-Val-L-leu-L-lys-p-nitroanilide HCl (S-2251). At 37 degrees C, maximum ami...

journal_title：Thrombosis and haemostasis

authors： Vasudevan J,Humphries JE,Gonias SL

更新日期：1993-04-01 00:00:00

abstract：:Current thrombolytic strategies have a number of important shortcomings including resistance to recanalization and development of acute reocclusion. The purpose of this study was to investigate whether the lysis of venous thrombi by streptokinase could be enhanced by SR 46349, a novel 5-HT2 receptor antagonist, or clo...

journal_title：Thrombosis and haemostasis

authors： Herbert JM,Bernat A,Sainte-Marie M,Dol F,Rinaldi M

更新日期：1993-03-01 00:00:00

abstract：:Herpes simplex virus (HSV) infection is histopathologically associated with vascular injury, fibrinoid necrosis and inflammatory cell infiltrates. We have previously shown in vitro that HSV infection of human umbilical vein endothelial cells (HUVEC) promotes a procoagulant phenotype manifest by the induction of tissue...

journal_title：Thrombosis and haemostasis

authors： Bok RA,Jacob HS,Balla J,Juckett M,Stella T,Shatos MA,Vercellotti GM

更新日期：1993-03-01 00:00:00

abstract：:Type I von Willebrand disease (vWD) Vicenza is a rare variant with autosomal dominant transmission, characterized by the presence of supranormal von Willebrand factor (vWF) multimers in plasma, similar to those normally found in endothelial cells and megakaryocytes. The patients have very low levels of plasma vWF cont...

journal_title：Thrombosis and haemostasis

authors： Randi AM,Sacchi E,Castaman GC,Rodeghiero F,Mannucci PM

更新日期：1993-02-01 00:00:00

abstract：:Plasma samples from 35 patients with colorectal cancer, 16 patients with pancreatic cancer and 46 patients with various cancers in the terminal stage were analysed for soluble plasma thrombomodulin with an ELISA method. At time of diagnosis and before primary treatment, the patients with colorectal cancer had normal p...

journal_title：Thrombosis and haemostasis

authors： Lindahl AK,Boffa MC,Abildgaard U

更新日期：1993-02-01 00:00:00

abstract：:Human platelets were separated into subpopulations using continuous flow electrophoresis, which uses electrophoretic mobility as the separative parameter. The platelets with the greatest electrophoretic mobility showed higher amounts of total sialic acid than the less electrophoretically mobile subpopulation (74.3 +/-...

journal_title：Thrombosis and haemostasis

authors： Crook M,Crawford N

更新日期：1993-01-11 00:00:00

abstract：:Fresh retracted clots are known to be poorly lysable by fibrinolytic agents. We have studied whether lysis of retracted clots could be enhanced by bulk transport in comparison to pure diffusion of plasma containing urokinase (400 IU/ml) into the clots. Cylindrical retracted blood clots were occlusively glued by a poly...

journal_title：Thrombosis and haemostasis

authors： Blinc A,Keber D,Lahajnar G,Stegnar M,Zidansek A,Demsar F

更新日期：1992-12-07 00:00:00

abstract：:We evaluated the effect of d-alpha-tocopherol (vitamin E) on the production of prostacyclin (PGI2) by cultured bovine aortic endothelial cells. Vitamin E at physiological doses significantly enhanced the production of PGI2 by aortic endothelial cells when added to the culture simultaneously with histamine, the Ca2+ io...

journal_title：Thrombosis and haemostasis

authors： Kunisaki M,Umeda F,Inoguchi T,Nawata H

更新日期：1992-12-07 00:00:00

abstract：:The significance of platelet beta-adrenoceptors for platelet responses to adrenergic stimuli in vivo and in vitro was studied in healthy volunteers. Low dose infusion of the beta-adrenoceptor agonist isoprenaline decreased platelet aggregability in vivo as measured by ex vivo filtragometry. Infusion of adrenaline, a m...

journal_title：Thrombosis and haemostasis

authors： Larsson PT,Wallén NH,Martinsson A,Egberg N,Hjemdahl P

更新日期：1992-12-07 00:00:00

abstract：:Congenitally abnormal fibrinogen Osaka III with the replacement of gamma Arg-275 by His was found in a 38-year-old female with no bleeding or thrombotic tendency. Release of fibrinopeptide(s) by thrombin or reptilase was normal, but her thrombin or reptilase time in the absence of calcium was markedly prolonged and th...

journal_title：Thrombosis and haemostasis

authors： Yoshida N,Imaoka S,Hirata H,Matsuda M,Asakura S

更新日期：1992-11-10 00:00:00

abstract：:The fibrinolytic system was studied in 96 patients with type I diabetes mellitus. Patients were grouped according to their degree of retinopathy; 38 patients with no evidence of retinopathy, 28 patients with background retinopathy and 30 patients with proliferative retinopathy. Thirty healthy individuals served as con...

journal_title：Thrombosis and haemostasis

authors： Ostermann H,Tschöpe D,Greber W,Meyer-Rüsenberg HW,van de Loo J

更新日期：1992-10-05 00:00:00

abstract：:The antithrombotic effect of the thromboxane A2 receptor antagonist, vapiprost, was compared with those of other antiplatelet drugs using an arterial thrombosis model which utilized photochemical reaction in the rat femoral artery. Vapiprost prolonged the time required to occlude the artery with thrombus and inhibited...

journal_title：Thrombosis and haemostasis

authors： Takiguchi Y,Wada K,Nakashima M

更新日期：1992-10-05 00:00:00

abstract：:Both the HEPTEST and amidolytic anti factor Xa assays are currently being used for heparin activity detection in plasma from patients receiving standard heparin or low molecular weight heparin (LMWH). In this study we have investigated the influence of recombinant and endogenous Tissue Factor Pathway Inhibitor (TFPI) ...

journal_title：Thrombosis and haemostasis

authors： Kristensen HI,Ostergaard PB,Nordfang O,Abildgaard U,Lindahl AK

更新日期：1992-09-07 00:00:00

abstract：:We studied the effect of purified immunoglobulins (Ig) from 21 patients with antiphospholipid antibodies (aPL) on factor Va degradation by activated protein C (aPC) on cultured human umbilical vein endothelial cells (HUVEC). Sera from patients were tested on an ELISA aPL assay to determine the isotype with aPL activit...

journal_title：Thrombosis and haemostasis

authors： Borrell M,Sala N,de Castellarnau C,Lopez S,Gari M,Fontcuberta J

更新日期：1992-09-07 00:00:00

abstract：:The aim of this study was to evaluate the thrombolytic activity of two hybrid plasminogen activators (HPAs) in a rabbit jugular vein thrombosis model. In the two HPAs the kringle-2 domain (K2tu-PA) or the finger and the kringle-2 domains (FK2tu-PA) of tissue-type plasminogen activator (t-PA) are linked to the catalyti...

journal_title：Thrombosis and haemostasis

authors： Agnelli G,Pascucci C,Colucci M,Nenci GG,Mele A,Bürgi R,Heim J

更新日期：1992-09-07 00:00:00